How does biotin work?

Biotin's general role as an enzymatic cofactor has also led to some research suggesting it may interact with glucose metabolism in the human body. As a general statement, it seems that in rodents with a higher circulating biotin level in their blood, the amount of insulin released in response to a glucose test is higher, leading to less elevation of glucose over time. This rodent evidence also reports that the higher glucose is not met with higher insulin resistance, suggesting a potentially beneficial role. Not too much evidence has been conducted in humans in regard to diabetes, with one study finding that intramuscular biotin was able to attenuate symptoms of neuropathy in three diabetic subjects.