Testosterone levels are assessed using a blood test. The cutoff for low testosterone differs between organizations, but most define it as a total testosterone level of <231–275 ng/dL.[1] Testosterone levels should be assessed early in the morning (between 7–11 a.m.) when peak levels occur, and in a fasted state. Two separate low testosterone measurements (preferably four weeks apart) along with clinical signs and symptoms of low testosterone are needed to be diagnosed with low testosterone.
How is low testosterone diagnosed?
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•Last Updated: May 16, 2025
Low testosterone is diagnosed through blood tests that measure testosterone levels; the tests are typically conducted in the morning while fasting. A diagnosis requires 2 separate low testosterone measurements, along with clinical signs and symptoms; low testosterone is defined as a total level that is less than 231 to 275 ng/dL.